{"332231":{"#nid":"332231","#data":{"type":"news","title":"3D Imaging of Transition Metals in the Zebrafish Embryo","body":[{"value":"\u003Cp\u003EA group of researchers including Chong Shin, an assistant professor in the School of Biology and Christoph Fahrni, a professor in the School of Chemistry and Biochemistry at Georgia Tech conducted a study to visualize the distribution of transition metals in the zebrafish embryo by employing synchrotron X-ray fluorescence (SXRF) microtomography. \u0026nbsp;\u003Cbr \/\u003E\u003Cbr \/\u003EBy combining the progressive lowering of temperature method (PLT) with femtosecond laser sectioning, a zebrafish embryo at 24 hours post fertilization was embedded, excised, and preserved in an X-ray compatible, transparent resin for tomographic elemental imaging. 3D distribution of zinc, iron, and copper was then reconstructed using the iterative maximum likelihood expectation maximization (MLEM) reconstruction algorithm based on a data set comprised of 60 projections, acquired with a step size of 2 \u03bcm during 100 hours of beam time. The volumetric elemental maps, which entail over 124 million individual voxels for each transition metal, demonstrated distinct elemental distributions correlated with characteristic anatomical features at this stage of embryonic development.\u003Cbr \/\u003E\u003Cbr \/\u003EThe study was published as an \u2018inside front cover\u2019 in the journal \u003Cem\u003EMetallomics\u003C\/em\u003E (Bourassa et al., 2014 Sep;6(9):1648-55. doi: 10.1039\/c4mt00121d). It has also been selected as one of the outstanding results by the management of the Advanced Photon Source (APS) sector where the work was done.\u003Cbr \/\u003E\u003Cbr \/\u003EThis project was supported by the \u003Cem\u003ENational Science Foundation (CHE-1306943)\u003C\/em\u003E, \u003Cem\u003Ethe National Institutes of Health (K01DK081351), and the Vasser Woolley Foundation\u003C\/em\u003E.\u003Cbr \/\u003E\u003Cbr \/\u003E\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"\u003Cp\u003EA group of researchers including Chong Shin, an assistant professor in the School of Biology and Christoph Fahrni, a professor in the School of Chemistry and Biochemistry at Georgia Tech conducted a study to visualize the distribution of transition metals in the zebrafish embryo by employing synchrotron X-ray fluorescence (SXRF) microtomography.\u003C\/p\u003E","format":"limited_html"}],"field_summary_sentence":[{"value":"A group of researchers conducted a study to visualize the distribution of transition metals in the zebrafish embryo by employing synchrotron X-ray fluorescence (SXRF) microtomography."}],"uid":"27245","created_gmt":"2014-10-08 11:35:40","changed_gmt":"2016-10-08 03:17:11","author":"Troy Hilley","boilerplate_text":"","field_publication":"","field_article_url":"","dateline":{"date":"2014-10-08T00:00:00-04:00","iso_date":"2014-10-08T00:00:00-04:00","tz":"America\/New_York"},"extras":[],"hg_media":{"332211":{"id":"332211","type":"image","title":"3D imaging of transition metals in the zebrafish embryo","body":null,"created":"1449245114","gmt_created":"2015-12-04 16:05:14","changed":"1475895044","gmt_changed":"2016-10-08 02:50:44","alt":"3D imaging of transition metals in the zebrafish embryo","file":{"fid":"200389","name":"metallomics.jpg","image_path":"\/sites\/default\/files\/images\/metallomics_0.jpg","image_full_path":"http:\/\/tlwarc.hg.gatech.edu\/\/sites\/default\/files\/images\/metallomics_0.jpg","mime":"image\/jpeg","size":528882,"path_740":"http:\/\/tlwarc.hg.gatech.edu\/sites\/default\/files\/styles\/740xx_scale\/public\/images\/metallomics_0.jpg?itok=F5Xv3Xky"}}},"media_ids":["332211"],"groups":[{"id":"1275","name":"School of Biological Sciences"}],"categories":[{"id":"146","name":"Life Sciences and Biology"}],"keywords":[{"id":"20691","name":"Chong Shin"},{"id":"363","name":"NSF"},{"id":"3084","name":"zebrafish"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[],"invited_audience":[],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}