{"343771":{"#nid":"343771","#data":{"type":"event","title":"MS Defense by Adam Blake Dunaway","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003EAdam Blake Dunaway\u003C\/strong\u003E\u003Cbr \/\u003E\u003Cbr \/\u003EDate: Thursday\u0026nbsp;Nov 20, 2014\u003Cbr \/\u003ETime: 1:30-3:30pm\u003Cbr \/\u003ELocation: \u0026nbsp;MoSE 2100F\u003Cbr \/\u003E\u003Cbr \/\u003E\u003Cbr \/\u003EAdvisor:\u003Cbr \/\u003EValeria T. Milam, PhD\u003Cbr \/\u003ESchool of Materials Science and Engineering\u003Cbr \/\u003EGeorgia Institute of Technology, GA\u003Cbr \/\u003E\u003Cbr \/\u003ECommittee:\u003Cbr \/\u003EJohnna S. Temenoff, PhD\u003Cbr \/\u003EDepartment of Biomedical Engineering\u0026nbsp;\u003Cbr \/\u003EGeorgia Institute of Technology, GA\u003Cbr \/\u003E\u003Cbr \/\u003EMeisha Shorner, PhD\u003Cbr \/\u003ESchool of Materials Science and Engineering\u003Cbr \/\u003EGeorgia Institute of Technology, GA\u003Cbr \/\u003E\u0026nbsp;\u003Cbr \/\u003E\u003Cstrong\u003ECharacterization of the Binding Activity of Immobilized DNA Aptamers for\u003C\/strong\u003E\u003Cbr \/\u003E\u003Cstrong\u003ENucleotide and Non-nucleotide Targets\u003C\/strong\u003E\u003Cbr \/\u003E\u003Cbr \/\u003EDeoxyribonucleic acid (DNA) aptamers are oligonucleotides with high\u003Cbr \/\u003Especificity and affinity for non-nucleotide targets ranging from molecular\u003Cbr \/\u003Especies to cellular proteins. Their high affinity, rapid synthesis, and\u003Cbr \/\u003Ethe ease with which they can be chemically modified to include convenient\u003Cbr \/\u003Echemical groups (e.g. amine group on 5\u0027 end) make them excellent adaptable\u003Cbr \/\u003Eligands for use in colloidal drug delivery vehicles for both uptake and\u003Cbr \/\u003Erelease of therapeutic agents. This work uses pre-identified aptamers for\u003Cbr \/\u003Evascular endothelial growth factor (VEGF) to investigate the design of one\u003Cbr \/\u003Esuch vehicle for controlled uptake and release of target therapeutics and\u003Cbr \/\u003Eanalyzes the ability of particle-immobilized aptamers to bind both\u003Cbr \/\u003Enucleotide and non-nucleotide targets. Aptamer sequences are immobilized\u003Cbr \/\u003Eon colloidal microspheres and binding activity of both the primary DNA and\u003Cbr \/\u003Eprotein targets are directly monitored using flow cytometry. Additionally,\u003Cbr \/\u003Ethe dual nature of aptamer-target binding is further investigated by\u003Cbr \/\u003Eevaluating the effects of simultaneous and serial incubation of the\u003Cbr \/\u003Eprimary targets. Finally, the ability to recover the functionality of the\u003Cbr \/\u003Eaptamer is evaluated after displacement of the primary DNA target through\u003Cbr \/\u003EDNA mediated interactions. \u0026nbsp;It has been shown that the nature of\u003Cbr \/\u003Eaptamer-target interactions are complex in nature, requiring optimization\u003Cbr \/\u003Efor each species incorporated into a delivery vehicle; however, partial\u003Cbr \/\u003Erecovery of aptamer functionality was achieved after hybridization with\u003Cbr \/\u003Ethe primary DNA target.\u0026nbsp;\u003Cbr \/\u003E\u003Cbr \/\u003E\u0026nbsp;\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":"Characterization of the Binding Activity of Immobilized DNA Aptamers for Nucleotide and Non-nucleotide Targets"}],"uid":"28077","created_gmt":"2014-11-10 15:00:04","changed_gmt":"2016-10-08 02:10:13","author":"Danielle Ramirez","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2014-11-20T12:30:00-05:00","event_time_end":"2014-11-20T14:30:00-05:00","event_time_end_last":"2014-11-20T14:30:00-05:00","gmt_time_start":"2014-11-20 17:30:00","gmt_time_end":"2014-11-20 19:30:00","gmt_time_end_last":"2014-11-20 19:30:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"109171","name":"MS Defense; graduate students"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78771","name":"Public"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}