{"663144":{"#nid":"663144","#data":{"type":"event","title":"PhD Proposal by Valerie Kay","body":[{"value":"\u003Cp\u003E\u003Cstrong\u003EValerie Kay\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EBioE PhD Proposal Presentation\u003C\/strong\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ETime and Date:\u003C\/strong\u003E\u0026nbsp;1:30 PM, Monday, November 21st, 2022\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ELocation: \u003C\/strong\u003EES\u0026amp;T L1120\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EVirtual Link:\u003C\/strong\u003E\u0026nbsp;\u003Ca href=\u0022https:\/\/gatech.zoom.us\/j\/92013582451\u0022\u003Ehttps:\/\/gatech.zoom.us\/j\/92013582451\u003C\/a\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EAdvisor:\u003C\/strong\u003E Corey Wilson, Ph.D. (Chemical and Biomolecular Engineering, Georgia Institute of Technology)\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003ECommittee Members:\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003EJohn Blazeck, Ph.D. (Chemical and Biomolecular Engineering, Georgia Institute of Technology)\u003C\/p\u003E\r\n\r\n\u003Cp\u003EJulie Champion, Ph.D. (Chemical and Biomolecular Engineering, Georgia Institute of Technology)\u003C\/p\u003E\r\n\r\n\u003Cp\u003EYury Chernoff, Ph.D. (Biological Sciences, Georgia Institute of Technology)\u003C\/p\u003E\r\n\r\n\u003Cp\u003EBrian Hammer, Ph.D. (Biological Sciences, Georgia Institute of Technology)\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u0026nbsp;\u003C\/p\u003E\r\n\r\n\u003Cp\u003E\u003Cstrong\u003EEngineering Transcriptional Programming to Develop Complete Decision Making and Memory in a Yeast Chassis Cell\u003C\/strong\u003E\u003C\/p\u003E\r\n\r\n\u003Cp\u003ESynthetic biology is a multidisciplinary approach to engineering new biological parts for beneficial purposes, such as developing complex genetic circuits. Transcription factors (TFs), which regulate transcription of targeted genes, are proficient at building these circuits. While transcriptional programming has been developed in prokaryotic chasses, there is growing interest in expanding these systems into higher-order eukaryotic cells, especially in the model yeast \u003Cem\u003ES.\u0026nbsp;cerevisiae\u003C\/em\u003E chassis. These cells have advantageous protein production and secretion capabilities, making them valuable for recombinant protein expression in biomanufacturing and for application as living therapeutics. Therefore, a modular set of TFs will be engineered for function in \u003Cem\u003ES. cerevisiae \u003C\/em\u003Eand used to build transcriptional programming, conferring this chassis with complete decision-making capabilities. Additionally, recombinase enzymes regulated by transcriptional programming will be used to build memory circuits that will enable the cells to permanently store decisions through inheritable changes in their genetics. To optimize these circuits, design rules for engineering allosteric communication of TFs \u003Cem\u003Ede novo \u003C\/em\u003Emust be developed. Therefore, the allosteric pathways of LacI anti-repressors will be mapped using deep-mutational scanning. By building and optimizing genetic circuits, complete decision making and memory systems will be built in \u003Cem\u003ES.\u0026nbsp;cerevisiae\u003C\/em\u003E, conferring complete intelligence to this higher-order eukaryotic chassis.\u003C\/p\u003E\r\n","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":"","field_summary_sentence":[{"value":"Engineering Transcriptional Programming to Develop Complete Decision Making and Memory in a Yeast Chassis Cell"}],"uid":"27707","created_gmt":"2022-11-14 16:28:33","changed_gmt":"2022-11-14 16:28:33","author":"Tatianna Richardson","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2022-11-21T13:30:00-05:00","event_time_end":"2022-11-21T15:00:00-05:00","event_time_end_last":"2022-11-21T15:00:00-05:00","gmt_time_start":"2022-11-21 18:30:00","gmt_time_end":"2022-11-21 20:00:00","gmt_time_end_last":"2022-11-21 20:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"groups":[{"id":"221981","name":"Graduate Studies"}],"categories":[],"keywords":[{"id":"102851","name":"Phd proposal"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1788","name":"Other\/Miscellaneous"}],"invited_audience":[{"id":"78761","name":"Faculty\/Staff"},{"id":"78771","name":"Public"},{"id":"78751","name":"Undergraduate students"}],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[],"email":[],"slides":[],"orientation":[],"userdata":""}}}