{"90291":{"#nid":"90291","#data":{"type":"event","title":"(10-1022) Prof. Yousif Shamoo, Rice University","body":[{"value":"\u003Cp\u003EProf. Yousif Shamoo, Rice University\n\u003C\/p\u003E\n\u003Cp\u003EEvolutionary fates within a microbial population: The essential role for protein folding during natural selection\n\u003C\/p\u003E\n\u003Cp\u003EBiochemistry Divisional Seminar Series\n\u003C\/p\u003E\n\u003Cp\u003ESystems biology can offer a great deal of insight into evolution by quantitatively linking complex properties such as protein structure, folding, and function to the fitness of an organism. Although the link between diseases such as Alzheimer\u0027s and misfolding is well appreciated,directly showing the importance of protein folding to success in evolution has been more difficult. We show here that predicting success during adaptation can depend critically on enzyme kinetic and folding models. We used a \u00e2\u0080\u0098weak link\u00e2\u0080\u0099 method to favor mutations to an essential, but maladapted, adenylate kinase gene within a microbial population that resulted in the identification of five mutants that arose nearly simultaneously and competed for success. Physicochemical characterization of these mutants showed that, although steady-state enzyme activity is important, success within the population is critically dependent on resistance to denaturation and aggregation. A fitness function based on in vitro measurements of enzyme activity,\u003Cbr \/\u003E\nreversible and irreversible unfolding, and the physiological context reproduces in vivo evolutionary fates in the population linking organismal adaptation to its physical basis.\n\u003C\/p\u003E\n\u003Cp\u003EFor more information contact \u003Ca href=\u0022mailto:loren.williams@chemistry.gatech.edu\u0022\u003EProf. Loren Williams\u003C\/a\u003E (404-894-9752).\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"Prof. Yousif Shamoo, Rice University\n\nEvolutionary fates within a microbial population: The essential \nrole for protein folding during natural selection\n\nBiochemistry Divisional Seminar Series","format":"limited_html"}],"field_summary_sentence":[{"value":"Prof. Yousif Shamoo, Rice University"}],"uid":"27275","created_gmt":"2010-08-10 00:00:00","changed_gmt":"2016-10-08 01:47:09","author":"Shirley Tomes","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2010-10-22T17:00:00-04:00","event_time_end":"2010-10-22T18:00:00-04:00","event_time_end_last":"2010-10-22T18:00:00-04:00","gmt_time_start":"2010-10-22 21:00:00","gmt_time_end":"2010-10-22 22:00:00","gmt_time_end_last":"2010-10-22 22:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"related_links":[{"url":"http:\/\/www.bioc.rice.edu\/~shamoo\/","title":"Prof. Yousif Shamoo, Rice University"}],"groups":[{"id":"85951","name":"School of Chemistry and Biochemistry"}],"categories":[],"keywords":[{"id":"919","name":"Biochemistry"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cstrong\u003EShirley Tomes\u003C\/strong\u003E\u003Cbr \/\u003EChemistry \u0026amp; Biochemistry\u003Cbr \/\u003E\u003Ca href=\u0022http:\/\/www.gatech.edu\/contact\/index.html?id=st81\u0022\u003EContact Shirley Tomes\u003C\/a\u003E\u003Cbr \/\u003E\u003Cstrong\u003E404-894-0591\u003C\/strong\u003E","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}