{"91811":{"#nid":"91811","#data":{"type":"event","title":"(09-1016) Prof. Mitchell Winnik, University of Toronto","body":[{"value":"\u003Cp\u003EProf. Mitchell Winnik, University of Toronto\n\u003C\/p\u003E\n\u003Cp\u003EMetal-chelating polymers and polymernanoparticles designed for use in bioassays\n\u003C\/p\u003E\n\u003Cp\u003EWe are interested in bioassays based upon inductively coupled plasma mass spectrometry [ICP-MS], a technique widely used in mining and metallurgy for its ability to measure quantitatively, with high sensitivity, the presence of different metals in a sample. In analogy with fluorescence based bioassays, where one uses quantum dots or fluorescent dyes as labels, we use lanthanide (Ln) metal ions as labels. For example, by labeling antibodies with metal-chelating polymers and different Ln ions, we have been able to carry out highly multiplexed assays for phenotyping human leukemia cells. I will briefly describe the new technique of mass cytometry, which allows us to quantify 20 biomarkers on a cell-by-cell basis. Then I will describe polystyrene nanoparticles carrying ca 2000 Ln ions and their use in assays of cell adhesion. Then I will present the synthesis of core-shell polymer nanogels containing much higher amounts of metal ions in the form of doped LaF3 nanoparticles. These can be taken up by a variety of cells by non-specific endocytosis. They become incorporated into endosomes, where they appear not to have any effect on cell activity. The long term goal this nanoparticle project is to be able to label cells such as stem cells in order to monitor their differentiation.   \n\u003C\/p\u003E\n\u003Cp\u003EFor more information contact \u003Ca href=\u0022mailto:lyon@chemistry.gatech.edu\u0022\u003EProf. L. Andrew Lyon\u003C\/a\u003E (404-894-4090).\u003C\/p\u003E","summary":null,"format":"limited_html"}],"field_subtitle":"","field_summary":[{"value":"Prof. Mitchell Winnik, University of Toronto\n\nMetal-chelating polymers and polymernanoparticles designed for use in bioassays","format":"limited_html"}],"field_summary_sentence":[{"value":"Prof. Mitchell Winnik, University of Toronto"}],"uid":"27275","created_gmt":"2009-06-26 00:00:00","changed_gmt":"2016-10-08 01:48:59","author":"Shirley Tomes","boilerplate_text":"","field_publication":"","field_article_url":"","field_event_time":{"event_time_start":"2009-10-16T12:00:00-04:00","event_time_end":"2009-10-16T13:00:00-04:00","event_time_end_last":"2009-10-16T13:00:00-04:00","gmt_time_start":"2009-10-16 16:00:00","gmt_time_end":"2009-10-16 17:00:00","gmt_time_end_last":"2009-10-16 17:00:00","rrule":null,"timezone":"America\/New_York"},"extras":[],"related_links":[{"url":"http:\/\/www.chem.utoronto.ca\/ppl\/faculty_profile.php?id=74","title":"Prof. Mitchell Winnik, University of Toronto"}],"groups":[{"id":"85951","name":"School of Chemistry and Biochemistry"}],"categories":[],"keywords":[{"id":"919","name":"Biochemistry"}],"core_research_areas":[],"news_room_topics":[],"event_categories":[{"id":"1795","name":"Seminar\/Lecture\/Colloquium"}],"invited_audience":[],"affiliations":[],"classification":[],"areas_of_expertise":[],"news_and_recent_appearances":[],"phone":[],"contact":[{"value":"\u003Cstrong\u003EShirley Tomes\u003C\/strong\u003E\u003Cbr \/\u003EChemistry \u0026amp; Biochemistry\u003Cbr \/\u003E\u003Ca href=\u0022http:\/\/www.gatech.edu\/contact\/index.html?id=st81\u0022\u003EContact Shirley Tomes\u003C\/a\u003E\u003Cbr \/\u003E\u003Cstrong\u003E404-894-0591\u003C\/strong\u003E","format":"limited_html"}],"email":[],"slides":[],"orientation":[],"userdata":""}}}